Medium 199/TCM 199 500mL
Description
Medium 199, With Earles salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture 500mL.
General description
Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.
Reconstitution
Supplement with 0.1 g/L L-glutamine.
| components | phenol red: yesNaHCO3: yesHEPES: noL-glutamine: noEarle’;s salts (5% CO2): yessodium pyruvate: no |
| form | liquid |
| impurities | endotoxin, tested |
| pH | 2.5-4 (25 °C, 209 g/L) |
| Quality Level | 400 |
| shipped in | ambient |
| sterility | sterile-filtered |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
Sigma Aldrich
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