HOT FIREPol® Blend Master Mix Ready to Load

د.إ 2,262.10
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Description

Ready to load hot-start PCR master mix for more demanding PCR assays

  • increased yield, sensitivity and specificity
  • up to 5x higher fidelity
  • suitable for templates up to 5 kb
  • reduced primer dimer formation
  • load directly on gel after PCR
  • MgCl2 (mM) final concentration 1.5
  • 5 x 1ml | 1250 rxn

Description

HOT FIREPol® Blend Master Mix Ready to Load is a premixed ready-to-use solution containing all reagents required for PCR (except template, primers, and water), an additional compound needed for direct loading onto an agarose gel, and two tracking dyes (blue and yellow) that allow monitoring progress during electrophoresis.

HOT FIREPol® Blend Master Mix contains two carefully optimized enzymes – HOT FIREPol® DNA polymerase and a proofreading polymerase. This enzyme blend has both the 5’→ 3’ exonuclease activity as well as the 3’→ 5’ proofreading activity. HOT FIREPol® Blend Master Mix exhibits an increased fidelity (up to fivefold) compared to regular Taq polymerase.

In addition, the mix also allows the amplification of longer fragments.

Generated PCR products are compatible with blunt-end and T/A cloning procedures.

HOT FIREPol® Blend Master Mix exhibits an increased fidelity (up to fivefold) compared to regular Taq polymerase.

Recommendations

We recommend using HOT FIREPol® Blend Master Mix Ready to Load in any PCR application that will be visualized by agarose gel electrophoresis and ethidium bromide staining.

HOT FIREPol® Blend Master Mix Ready to Load is not recommended for use in applications where spectrophotometric measurements (absorbance or fluorescence) are necessary because yellow and blue dyes can interfere with these applications.

Properties

Concentration: 5x

Hot-start: yes, initial activation in 12-15 min

Ready to load: yes

Fidelity: 5 x Taq

Cloning Type: T/A cloning and Blunt-end cloning

Amplicon Size: up to 5 kb

Mix Components

HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start

Proofreading enzyme: to enhance fidelity

5x Blend Master Mix Buffer with 7. 5 mM MgCl2: 1x PCR solution – 1.5 mM MgCl2

1 mM dNTPs of each: 1x PCR solution – 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP

Bovine serum albumin (BSA) to enhance PCR reaction

Blue dye: migration equivalent to 3.5-4.5 kb DNA fragment

Yellow dye: migration rate in excess of primers in 1% agarose gel: <35-45 bp

SOLIS BIODYNE

Solis BioDyne has been developing and producing life science reagents since 1995, having become one of the leading reagent suppliers in Europe today. High standards for production and service have made Solis BioDyne a trusted trademark worldwide. Solis BioDyne DNA polymerases, PCR Master Mixes, qPCR Mixes and reverse transcription reagents are used by a quickly growing number of customers across the globe, including top research institutes and biotech-companies. Solis BioDyne has partners in both private and state sectors, with cooperation projects ranging from OEM production to scientific research.
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