For in vitro use only!
Unit Definition: One unit is the amount of enzyme required to completely digest 1 μg of Lambda DNA (113 sites) in 1 hour in a total reaction volume of 50 μl. Enzyme activity was determined in the recommended reaction buffer.
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Form: liquid (Supplied in 10 mM Tris-HCl pH 7.4, 50 mM KCl, 0.1 mM EDTA, 200 μg/ml BSA and 50 % [v/v] glycerol)
Concentration: 10 units/μl
Source: Rhodopseudomonas sphaeroides
Supplied with: 10x Universal Buffer (UB)
Recommended 50 μl assay
|5 μl||10x Universal Buffer (UB)|
|1 μg||pure DNA1 or PCR product2|
|fill up to 50 μl||PCR grade water|
Reaction Buffer Compatibility:
Our restriction enzymes are fully compatible to restrictases and buffer systems from other manufacturers and can be used along in double digestions. To obtain best results, consult the corresponding manuals of all involved products.
Ligation and recutting:
After 10-fold overdigestion with RsaI, >95% of the DNA fragments can be ligated and recut with this enzyme.
No Inhibition: dam, dcm
Inhibition (Blocked by some combinations of overlapping): CpG