PCR Master Mix for routine applications
- suitable for templates up to 5 kb
- reaction set-up and shipment without dry ice
- all-in-one master mix format reduces pipetting errors and saves time
- amplicons are suitable for Sanger sequencing post-PCR
Optimized ready-to-use PCR master mix for routine PCR assays. This master mix contains thermostable Taq polymerase FIREPol®, MgCl2, dNTPs, and buffer with detergent. You just need to add a template, primers, and water.
We recommend using FIREPol® Master Mix without ready to load feature in all PCR applications where spectrophotometric measurements (absorbance or fluorescence) are necessary post-PCR because loading dyes can interfere with these applications.
Ready to load: no
Fidelity: same as Taq
Cloning Type: T/A Cloning
Amplicon Size: up to 5 kb
FIREPol® DNA polymerase: highly processive. thermostable DNA polymerase
5x Reaction Buffer B: 0.4 M Tris-HCl, 0.1 M (NH4)2SO4, 0.1% w/v Tween-20
7.5 mM MgCl2: 1x PCR solution – 1.5 mM MgCl2)
1 mM dNTPs of each: 1x PCR solution – 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP