5x FIREPol® Master Mix 5X 1 ml | 1250 rxn

د.إ 1,690.10
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04-11-00125-5

Description

PCR Master Mix for routine applications

  • suitable for templates up to 5 kb
  • reaction set-up and shipment without dry ice
  • all-in-one master mix format reduces pipetting errors and saves time
  • amplicons are suitable for Sanger sequencing post-PCR

Optimized ready-to-use PCR master mix for routine PCR assays. This master mix contains thermostable Taq polymerase FIREPol®, MgCl2, dNTPs, and buffer with detergent. You just need to add a template, primers, and water.

We recommend using FIREPol® Master Mix without ready to load feature in all PCR applications where spectrophotometric measurements (absorbance or fluorescence) are necessary post-PCR because loading dyes can interfere with these applications.

Properties

Concentration: 5x

Hot-start: no

Ready to load: no

Fidelity: same as Taq

Cloning Type: T/A Cloning

Amplicon Size: up to 5 kb

Mix Components

FIREPol® DNA polymerase: highly processive. thermostable DNA polymerase

5x Reaction Buffer B: 0.4 M Tris-HCl, 0.1 M (NH4)2SO4, 0.1% w/v Tween-20

7.5 mM MgCl21x PCR solution – 1.5 mM MgCl2)

1 mM dNTPs of each:  1x PCR solution – 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP

SOLIS BIODYNE

Solis BioDyne has been developing and producing life science reagents since 1995, having become one of the leading reagent suppliers in Europe today. High standards for production and service have made Solis BioDyne a trusted trademark worldwide. Solis BioDyne DNA polymerases, PCR Master Mixes, qPCR Mixes and reverse transcription reagents are used by a quickly growing number of customers across the globe, including top research institutes and biotech-companies. Solis BioDyne has partners in both private and state sectors, with cooperation projects ranging from OEM production to scientific research.
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